The Jensen Lab @ Michigan

Automation Hardware

Our lab uses state-of-the-art hardware to tackle large and complex biological problems. Our current automation system includes the following instruments.

Measurement & Detection

	Cytation 10 Automated Microscope/Multimode Plate Reader Agilent 20x-40x confocal imaging (60 um spinning disk) 4x-40x phase contrast imaging 1.25x-40x brightfield imaging Sony CMOS, 16-bit grayscale camera 6- to 1536-well plate reading monochromator fluorescence (variable bandwidth) filter-dichroic fluorescence monochromator UV-Vis absorbance luminescence aerobic & anaerobic incubation dual reagent injectors
	Cytation 5 Multimode Plate Reader Agilent 6- to 1536-well plate reading monochromator fluorescence (variable bandwidth) filter-dichroic fluorescence monochromator UV-Vis absorbance luminescence aerobic & anaerobic incubation dual reagent injectors pH-XTRA glycolysis module
	Synergy H1 Multimode Plate Reader (x2) Agilent 6- to 384-well plate reading monochromator fluorescence (variable bandwidth) monochromator UV-Vis absorbance luminescence temperature control linear, orbital, and double orbital shaking
	Epoch 2 Plate Reader (x3) Agilent monochromator UV-Vis absorbance 6- to 384-well plate reading incubation and shaking automation compatible

Liquid Handling

	Tempest Liquid Handler (x2) Formulatrix 48 independent dispense nozzles 6 reagent capacity 0.2 - 5.0 ul dispense range automated plate stackers (25 plate capacity)
	Mantis Liquid Handler with LC3 Formulatrix 28 reagent capacity 0.1 - 5.0 ul dispense range
	Cobra Nano Dispenser Hudson Robotics 0.2-100 uL aspiration non-contact dispensing 2 plate positions on-deck tip cleaning
	Nimbus Liquid Handler Hamilton 8 deck positions 4 head spanning pipettor plate gripper
	MultiFlo FX Multimode Washer/Dispenser BioTek 2 peristaltic pumps 2 syringe pumps Random Access Dispense (RAD) module Automated Media Exchange (AMX) module Wash module for magnetic bead purification
	OT-2 Liquid Handler (x2) OpenTrons programmable aspiration and dispensing single and 8-channel pipette heads
	Platemaster 96-Channel Pipette (x3) Gilson 2-220 uL capacity 96 or 384 well plate formats 4 deck positions

Movement & Incubation

	BioSpa 8 Robotic Incubator (x2) Agilent 8 plate capacity temperature, CO₂, and O₂ control integrates with the Cytation 10, 5, Synergy Neo, and Epoch plate readers
	BioStack 2WR Plate Handler (x2) Agilent 50 plate capacity 30 second plate change time
	PlateCrane EX Robotic Arm (x2) Hudson Robotics 345° travel 18" horizontal reach removable plate stacks
	RapidPick SP Colony Picker Hudson Robotics single pin with sterilization up to 400 colonies/hour plate re-arraying

Molecular Biology

	QuantStudio 5 Real-Time PCR System Applied Biosystems 96 well 0.2 mL labware 6 temperature zones 6 decoupled channels + white LED
	ProFlex PCR System Applied Biosystems 3 x 32 well 0.2 mL labware 6.0 °C/s ramp rate
	SimpliAmp Thermocycler Applied Biosystems 96 well 0.2 mL labware 4.0 °C/s ramp rate
	Bioanalyzer Automated Electrophoresis Instrument Agilent DNA/RNA sizing 12 samples/chip